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Purification and characterisation of a hypoglycemic peptide from Momordica Charantia L. Var. abbreviata Ser. by Xiaoqing Yuan; Xiaohong Gu; Jian Tang is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Purification and characterisation of a hypoglycemic peptide from Momordica Charantia L. Var. abbreviata Ser. about?
A water-soluble peptide MC2-1-5 from Momordica charantia L. Var. Abbreviata Ser., with hypoglycemic effect, was purified by ultrafiltration, gel filtration chromatography and reverse-phase high performance liquid chromatography (RP-HPLC). The infrared (IR) spectra showed characteristic absorption peaks and the molecular mass of MC2-1-5 was found to be 3405.5174Da by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The sequence of its first 10 N-terminal amino acids was GHPYYSIKKS as determined by a protein sequencer. MC2-1-5 reduced the blood glucose level in alloxan-induced diabetic mice by 61.70% and 69.18% at 2 and 4h, respectively, after oral administration at a dose of 2mg/kg. The oral glucose tolerance test (OGTT) showed MC2-1-5 produced a reduction of 25.50%, 39.62% and 41.74% in blood glucose level after 1, 2 and 3h, respectively, of oral administration compared with a diabetic control.
Who reads Purification and characterisation of a hypoglycemic peptide from Momordica Charantia L. Var. abbreviata Ser.?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Xiaoqing Yuan; Xiaohong Gu; Jian Tang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)
- Published
- 2008
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)