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This document describes a study that used the killing of the nematode Caenorhabditis elegans by Pseudomonas aeruginosa to identify virulence factors important for P. aeruginosa pathogenesis in mammals. Eight P. aeruginosa mutants that were defective in C. elegans killing were isolated and at least five also showed reduced virulence in mice. Three mutants corresponded to known virulence genes, while three corresponded to genes not previously known to be involved in pathogenesis. This validates the use of a C

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chai236

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EN