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Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.) by S. Rueb; M. Leneman; R. A. Schilperoort; L. A. M. Hensgens is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.) about?

To obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus four published methods of callus induction and regeneration were compared. Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice (Oryza sativa L.). The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. A limited humidity and an optimal aeration of the culture vessels enhanced the frequency of embryogenesis and plant regeneration. A method described by was found to be the most efficient and was slightly modified. As a result 98% of the T309 embryos formed callus, of which 63% regenerated into plants. Each callus yielded an average of 6 plants. Plant morphology, fertility and seed set of the regenerants were found to be normal.

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Author

S. Rueb; M. Leneman; R. A. Schilperoort; L. A. M. Hensgens

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6857)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)