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Determination of the Antibacterial Ofloxacin in Human Urine and Serum Samples by Solid-phase Spectrofluorimetry by Oscar Ballesteros; José Luis Vı́lchez; Alberto Navalón is a Chemistry article available to read on EtoBox.

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A method for the determination of trace amounts of ofloxacin has been developed, based on solid-phase spectrofluorimetry. The relative fluorescence intensity of ofloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1-mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 294 and 494 nm, respectively. The linear concentration range of application was 0.5-16.0 ng ml(-1) of ofloxacin, with a relative standard deviation of 1.1% (for a level of 8.0 ng ml(-1)) and a detection limit of 0.14 ng ml(-1). The method was applied to the determination of ofloxacin in human urine and serum samples. It was validated applying the standard addition methodology and using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.

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Author

Oscar Ballesteros; José Luis Vı́lchez; Alberto Navalón

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)