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Can I read Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys ( Macaca fascicularis ) on EtoBox?
Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys ( Macaca fascicularis ) by A.H. Feradis; D. Pawitri; I.K. Suatha; M.R. Amin; T.L. Yusuf; D. Sajuthi; I.N. Budiarsa; E.S. Hayes is a Immunology and Microbiology article available to read on EtoBox.
What is Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys ( Macaca fascicularis ) about?
We have examined the motility, morphology, and cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (__Macaca fascicularis__). At collection, epididymal sperm (23×10^6^±4×10^6^ sperm/sample; 611×10^6^±116×10^6^ sperm/ml; n=18) were alive (79±2%), motile (67±2%), and exhibited intact membranes (65±2%). Sperm maintained at room temperature in handling medium exhibited decreased motility over time, but head‐to‐head agglutination was limited. Tris egg‐yolk extender containing 6% glycerol and dimethylsulfoxide (DMSO) did not significantly affect functional morphology, whereas extender containing propanediol significantly reduced motility, survival, and membrane integrity. Cryostorage reduced all measures of functional morphology independent of cryoprotectant. Post‐thaw motility was superior for glycerol and DMSO compared to propanediol. Variation in glycerol concentration (4, 6, and 8%) produced equivocal effects on sperm functional morphology post‐thaw. Needle biopsy may be a useful technique for laboratory and field‐based collection of spermatozoa from nonhuman primates.
Who reads Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys ( Macaca fascicularis )?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- A.H. Feradis; D. Pawitri; I.K. Suatha; M.R. Amin; T.L. Yusuf; D. Sajuthi; I.N. Budiarsa; E.S. Hayes
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; S. Karger AG (ISSN 0047-2565)
- Published
- 2001
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)