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Amylolytic activity of IgG and sIgA immunoglobulins from human milk by Andrew N Savel'ev; Tat'yana G Kanyshkova; Anna A Kulminskaya; Valentina N Buneva; Elena V Eneyskaya; Michael V Filatov; Georgy A Nevinsky; Kirill N Neustroev is a Medicine article available to read on EtoBox.

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Background: New natural amylolytic abzymes Abs for catalytically active antibodies from human milk have been identified and investigated. Methods: The amylolytic activity of human milk autoantibodies was studied by TLC and HPLC techniques analyzing the hydrolysis of maltooligosaccharides with different degrees of polymerization and of 4-nitrophenyl Ž . 4,6-O-ethylidene-a-D-maltoheptaoside EPS . IgG and sIgA fractions were isolated from human milk by affinity chromatography. After SDS-PAGE preparation of native IgG and sIgA and their renaturation, the amylolytic activity was in-gel assayed. Results: All electrophoretically homogeneous preparations of IgG and its Fab fragments as well as sIgA antibodies possessed a-amylolytic activity. The specific activities of these catalytic antibodies varied in the range from 1.83 up to 3.33 katrkg, which is about one order of magnitude higher than that for IgGs from the sera of cancer patients. IgG and sIgA fractions showed Michaelis constants for hydrolysis of 4-nitrophenyl 4,6-O-ethylidene-a-D-maltoheptaoside in the range of 10 y4 Mrl. Fractions of autoantibodies from different donors exhibited different modes of action in hydrolysis of maltoo

Who reads Amylolytic activity of IgG and sIgA immunoglobulins from human milk?

Author

Andrew N Savel'ev; Tat'yana G Kanyshkova; Anna A Kulminskaya; Valentina N Buneva; Elena V Eneyskaya; Michael V Filatov; Georgy A Nevinsky; Kirill N Neustroev

Publisher

Elsevier BV

Published

2001

Language

EN

Field

Medicine (Health Sciences)