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Can I read Isolation, Characterization, and in Vitro Propagation of Infantile Hemangioma Stem Cells and an in Vivo Mouse Model on EtoBox?
Isolation, Characterization, and in Vitro Propagation of Infantile Hemangioma Stem Cells and an in Vivo Mouse Model by Dan Xu; Teresa M O; Archil Shartava; Taylor C Fowles; Jianchang Yang; Louis M Fink; David C Ward; Martin C Mihm; Milton Waner; Yupo Ma is a Medicine article available to read on EtoBox.
What is Isolation, Characterization, and in Vitro Propagation of Infantile Hemangioma Stem Cells and an in Vivo Mouse Model about?
## Background Infantile hemangiomas (IH) are the most common benign tumors of infancy. The typical clinical course consists of rapid growth during the first year of life, followed by natural and gradual involution over a multi-year time span through unknown cellular mechanisms. Some tumors respond to medical treatment with corticosteroids or beta-blockers, however, when this therapy fails or is incomplete, surgical extirpation may be necessary. Noninvasive therapies to debulk or eliminate these tumors would be an important advance. The development of an __in vitro__ cell culture system and an animal model would allow new insights into the biological processes involved in the development and pathogenesis of IH. ## Results We observed that proliferative stage IH specimens contain significantly more SALL4+ and CD133+ cells than involuting tumors, suggesting a possible stem cell origin. A tumor sphere formation assay was adapted to culture IH cells __in vitro__. Cells in IH tumor spheres express GLUT1, indicative of an IH cell of origin, elevated levels of VEGF, and various stem/progenitor cell markers such as SALL4, KDR, Oct4, Nanog and CD133. These cells were able to self-renew and d
Who reads Isolation, Characterization, and in Vitro Propagation of Infantile Hemangioma Stem Cells and an in Vivo Mouse Model?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Dan Xu; Teresa M O; Archil Shartava; Taylor C Fowles; Jianchang Yang; Louis M Fink; David C Ward; Martin C Mihm; Milton Waner; Yupo Ma
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2008-; Springer Science and Business Media LLC (ISSN 1756-8722)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)