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Transcriptomic and functional analysis of ANGPTL4 overexpression in pancreatic cancer nominates targets that reverse chemoresistance by Emily R. Gordon; Carter A. Wright; Mikayla James; Sara J. Cooper is a Medicine article available to read on EtoBox.

What is Transcriptomic and functional analysis of ANGPTL4 overexpression in pancreatic cancer nominates targets that reverse chemoresistance about?

## Background Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers based on five-year survival rates. Genes contributing to chemoresistance represent novel therapeutic targets that can improve treatment response. Increased expression of __ANGPTL4__ in tumors correlates with poor outcomes in pancreatic cancer. ## Methods We used statistical analysis of publicly available gene expression data (TCGA-PAAD) to test whether expression of __ANGPTL4__ and its downstream targets, __ITGB____4__ and __APOL1__, were correlated with patient survival. We measured the impact of __ANGPTL4__ overexpression in a common pancreatic cancer cell line, MIA PaCa-2 cells, using CRISPRa for overexpression and DsiRNA for knockdown. We characterized global gene expression changes associated with high levels of __ANGPTL4__ and response to gemcitabine treatment using RNA-sequencing. Gemcitabine dose response curves were calculated on modified cell lines by measuring cell viability with CellTiter-Glo (Promega). Impacts on cell migration were measured using a time course scratch assay. ## Results We show that __ANGPTL4__ overexpression leads to in vitro resistance to gemcitabine and reduced sur

Who reads Transcriptomic and functional analysis of ANGPTL4 overexpression in pancreatic cancer nominates targets that reverse chemoresistance?

Author

Emily R. Gordon; Carter A. Wright; Mikayla James; Sara J. Cooper

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Medicine (Health Sciences)