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Transitional CpG methylation between promoters and retroelements of tissue-specific genes during human mesenchymal cell differentiation by Moo-Il Kang; Hye-Soo Kim; Yu-Chae Jung; Young-Ho Kim; Seung-Jin Hong; Mi-Kyoung Kim; Ki-Hyun Baek; Chun–Choo Kim; Mun-Gan Rhyu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract In general, methylation of the promoter regions is inversely correlated with gene expression. The transitional CpG area between the promoter‐associated CpG islands and the nearby retroelements is often methylated in a tissue‐specific manner. This study analyzed the relationship between gene expression and the methylation of the transitional CpGs in two human stromal cells derived from the bone marrow (BMSC) and adipose tissue (ATSC), both of which have a multilineage differentiation potential. The transitional CpGs of the osteoblast‐specific (RUNX2 and BGLAP), adipocyte‐specific (PPARγ2), housekeeping (CDKN2A and MLH1), and mesenchyme‐unrelated (RUNX3) genes were examined by methylation‐specific PCR. The expression of each gene was measured using reverse‐transcription PCR analysis. The RUNX2, BGLAP, and CDKN2A genes in the BMSC, and the PPARγ2 gene in the ATSC exhibited hypomethylation of the transitional CpGs along with the strong expression. The CpG island of RUNX3 gene not expressed in both BMSC and ATSC was hypermethylated. Transitional hypomethylation of the MLH1 gene was accompanied by the higher expression in the BMSC than in the ATSC. The weakly methylated CpGs
Who reads Transitional CpG methylation between promoters and retroelements of tissue-specific genes during human mesenchymal cell differentiation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Moo-Il Kang; Hye-Soo Kim; Yu-Chae Jung; Young-Ho Kim; Seung-Jin Hong; Mi-Kyoung Kim; Ki-Hyun Baek; Chun–Choo Kim; Mun-Gan Rhyu
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)