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Double resistance to ganciclovir and foscarnet of four human cytomegalovirus strains recovered from AIDS patients by Antonella Sarasini; Fausto Baldanti; Milena Furione; Elena Percivalle; Roberto Brerra; Maria Barbi; Giuseppe Gerna is a Medicine article available to read on EtoBox.

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## Abstract Four human cytomegalovirus (HCMV) isolates from four different AIDS patients treated with both ganciclovir and foscarnet and not responding clinically to antiviral treatment, were studied in order to verify the occurrence of double resistance to both drugs, and to define whether single or multiple HCMV strains could be responsible for the double resistance. Peripheral blood leukocytes (PBL), the relevant conventional viral isolates, and plaque‐purified strains from all four patients were examined by antiviral drug susceptibility testing by an immediate‐early antigen plaque reduction assay and by restriction fragment length polymorphism (RFLP) analysis using polymerase chain reaction (PCR)‐amplified multiple genome regions and endonucleases. All four HCMV strains had a high level of resistance to both ganciclovir and foscarnet. A single HCMV strain was shown to be responsible for the dual resistance in each patient. HCMV strain identity and uniqueness were shown for each of the four patients in blood samples, viral isolates, and plaque‐purified strains. In addition, in two patients the same single HCMV strain shifted progressively from drug sensitivity to ganciclovir and

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Author

Antonella Sarasini; Fausto Baldanti; Milena Furione; Elena Percivalle; Roberto Brerra; Maria Barbi; Giuseppe Gerna

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

1995

Language

EN

ISBN

9781890470302

Field

Medicine (Health Sciences)