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What is Enzymatic Assay for Pyruvate Dehydrogenase about?
This document describes an enzymatic assay to measure the activity of pyruvate dehydrogenase. The assay works by measuring the conversion of pyruvate to acetyl-CoA, which is coupled to the reduction of NAD+ to NADH. This reaction is monitored spectrophotometrically by measuring the increase in absorbance at 340nm due to NADH production. The document provides detailed information on reagents, concentrations, calculations, and procedures for performing the continuous spectrophotometric assay.
- Author
- ADanAlfa
- Language
- EN