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Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine) by In-Kyu Park; Jurate Lasiene; Shinn-Huey Chou; Philip J. Horner; Suzie H. Pun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine) about?

## Abstract ## Background The development of minimally invasive, non‐viral gene delivery vehicles for the central nervous system (CNS) is an important technology goal in the advancement of molecular therapies for neurological diseases. One approach is to deliver materials peripherally that are recognized and retrogradely transported by motor neurons toward the CNS. Tet1 is a peptide identified by Boulis and coworkers to possess the binding characteristics of tetanus toxin, which interacts specifically with motor neurons and undergoes fast, retrograde delivery to cell soma. In this work, Tet1‐poly(ethylenimine) (Tet1‐PEI) was synthesized and evaluated as a neurontargeted delivery vehicle. ## Methods Tet1‐PEI and NT‐PEI (neurotensin‐PEI) were synthesized and complexed with plasmid DNA to form polyplexes. Polyplexes were assessed for binding and uptake in differentiated neuron‐like PC‐12 cells by flow cytometry and confocal microscopy. In order to determine gene delivery efficiency, polyplexes were exposed to PC‐12 cells at various stages of differentiation. Targeted binding of polyplexes with primary neurons was studied using dorsal root ganglion cells. ## Results Tet1‐PEI and NT‐PEI

Who reads Neuron-specific delivery of nucleic acids mediated by Tet1-modified poly(ethylenimine)?

Author

In-Kyu Park; Jurate Lasiene; Shinn-Huey Chou; Philip J. Horner; Suzie H. Pun

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)