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Identification of β‐lactoglobulin‐derived peptides and class II HLA molecules recognized by T cells from patients with milk allergy by R. Inoue; S. Matsushita; H. Kaneko; S. Shinoda; H. Sakaguchi; Y. Nishimura; N. Kondo is a Medicine article available to read on EtoBox.

What is Identification of β‐lactoglobulin‐derived peptides and class II HLA molecules recognized by T cells from patients with milk allergy about?

**Background** Cow's milk allergy impairs the health and development of many infants since it deprives them of adequate nutrition. Cow's milk fractions contain many allergens, and β‐lactoglobulin (BLG) is one of the major allergens. **Objective** The purpose of this study was to determine T cell epitopes, antigen‐presenting molecules and cytokine production by T cells in relation to BLG. The results can provide new therapeutic possibilities of using analogue peptides of BLG for infants with cow's milk allergy. **Methods** Using a mixture of a panel of overlapping synthetic peptides that cover the entire BLG molecule, we established polyclonal BLG‐specific short‐term T cell lines and clones from peripheral blood mononuclear cells of four patients with allergy to cow's milk carrying most of the common human leucocyte antigen (HLA) haplotypes seen in the Japanese population. We then identified the T cell epitopes and antigen‐presenting molecules, and measured the production of cytokines interleukin (IL)‐4, IL‐5 and interferon‐γ in the culture supernatants. **Results** The T cell lines established from the four patients responded to seven different peptides. Three of the peptides stimu

Who reads Identification of β‐lactoglobulin‐derived peptides and class II HLA molecules recognized by T cells from patients with milk allergy?

Author

R. Inoue; S. Matsushita; H. Kaneko; S. Shinoda; H. Sakaguchi; Y. Nishimura; N. Kondo

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0954-7894)

Published

2001

Language

EN

Field

Medicine (Health Sciences)