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Inhibition of Transforming Growth Factor-β Signaling in Normal Lung Epithelial Cells Confers Resistance to Ionizing Radiation by Anna Reeves; Marianna Zagurovskaya; Seema Gupta; Mohammed M. Shareef; Mohammed Mohiuddin; Mansoor M. Ahmed is a Medicine article available to read on EtoBox.

What is Inhibition of Transforming Growth Factor-β Signaling in Normal Lung Epithelial Cells Confers Resistance to Ionizing Radiation about?

Purpose: To address the functional role of radiation-induced transforming growth factor-␤ (TGF-␤) signaling in a normal epithelial background, we selected a spontaneously immortalized lung epithelial cell line derived from the normal lung tissue of a dominant-negative mutant of the TGF-␤ RII (⌬RII) transgenic mouse that conditionally expressed ⌬RII under the control of the metallothionein promoter (MT-1), and assessed this cell line's response to radiation. Methods and Materials: A spontaneously immortalized lung epithelial cell culture (SILECC) was established and all analyses were performed within 50 passages. Colony-forming and terminal transferase dUPT nick end labeling (TUNEL) assays were used to assess clonogenic inhibition and apoptosis, respectively. Western-blot analysis was performed to assess the kinetics of p21, bax, and RII proteins. Transforming growth factor-␤responsive promoter activity was measured using dual-luciferase reporter assay. Results: Exposure to ZnSO 4 inhibited TGF-␤ signaling induced either by recombinant TGF-␤1 or ionizing radiation. The SILECC, treated with either ZnSO 4 or neutralizing antibody against TGF-␤, showed a significant increase in radio-r

Who reads Inhibition of Transforming Growth Factor-β Signaling in Normal Lung Epithelial Cells Confers Resistance to Ionizing Radiation?

Author

Anna Reeves; Marianna Zagurovskaya; Seema Gupta; Mohammed M. Shareef; Mohammed Mohiuddin; Mansoor M. Ahmed

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)

Published

2007

Language

EN

Field

Medicine (Health Sciences)