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What is Enhancing CRISPR/Cas9 with tRNA System about?

This study presents a novel strategy to enhance the multiplex editing capability of the CRISPR/Cas9 system by utilizing an engineered endogenous tRNA-processing system to produce multiple guide RNAs (gRNAs) from a single polycistronic gene. The approach allows for efficient and precise processing of gRNAs, facilitating simultaneous targeting of multiple chromosomal sites in plants, particularly in stable transgenic rice. This method is expected to have broad applications in genome engineering across various

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db4djzqvr8

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EN