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High levels of the mitochondrial large ribosomal subunit protein 40 prevent loss of mitochondrial DNA in null mmf1 Saccharomyces cerevisiae cells by Rosita Accardi; Ellinor Oxelmark; Nicolas Jauniaux; Vito de Pinto; Antonio Marchini; Massimo Tommasino is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Members of the YERO57c/YJGFc/UK114 protein family have been identified in bacteria and eukaryotes. The budding yeast __Saccharomyces cerevisiae__ contains two different proteins of this family, Hmf1p and Mmf1p. We have previously shown that Mmf1p is a mitochondrial protein functionally related to its human homologue and able to influence the maintenance of mitochondrial DNA. Deletion of __Mmf1__ results in loss of the mitochondrial genome. Using a multicopy suppression approach, we have identified a protein of the mitochondrial large ribosomal subunit, MRPL40, which stabilizes mtDNA in Δ__mmf1__ cells. Overexpression of MRPL40 did not prevent loss of mtDNA in a mutant strain lacking the mitochondrial protein Abf2p. Thus, MRPL40 does not have a general effect on mtDNA stability, but it may be specific for the __mmf1__‐null strain. We also show that the Δ__mrpl40__ cells present a similar phenotype to the __mmf1__‐null strain, having reduced mtDNA stability and growth rate. Furthermore, we observed that ρ^+^Δ__mrpl40__ haploid cells can be obtained when tetrads are directly dissected on medium containing a non‐fermentable carbon source. Thus, replication and segregation o
Who reads High levels of the mitochondrial large ribosomal subunit protein 40 prevent loss of mitochondrial DNA in null mmf1 Saccharomyces cerevisiae cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rosita Accardi; Ellinor Oxelmark; Nicolas Jauniaux; Vito de Pinto; Antonio Marchini; Massimo Tommasino
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)