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Kaempferol Protects Mitochondria and Alleviates Damages Against Endotheliotoxicity Induced by Doxorubicin by Wu, Weiqi; Yang, Bin; Qiao, Yang; Zhou, Qing; He, Huan; He, Ming is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Kaempferol (Kae), a flavonoid, has been found in fruits and other vegetables, possesses many biological activities. 14-3-3 protein exerts protection on various types of injured tissues and cells. Doxorubicin (Dox) causes excessive reactive oxygen species (ROS) generation, which induces endotheliotoxicity and cardiotoxicity. We hypothesized that Kae could protect vascular endothelium by regulating 14-3-3γ or related pathways against Dox toxicity. HUVECs were established Dox-toxic injury models. Kae's effects were assessed with many physiological, enzymatic, cellular, and molecular biological indexes. Our results showed that Dox-induced damage in HUVECs were reduced through Kae to promote the expression of total protein 14-3-3γ and mitochondrial Bcl-2, phosphorylate Bad, increase cell viability, NO content, DDAHIIactivity, p-eNOS/eNOS ratio, and MMP levels, maintained NAD/NADH and GSH/GSSG balance, and decrease LDH and caspase-3 activities, ADMA content, ROS generation, mPTP openness, and apoptosis. Kae's effects were abolished with pAD/14-3-3γ-shRNA downregulating 14-3-3γ expression, or ABT-737 inhibiting Bcl-2 activity. This study demonstrated that Kae protected the vascular endoth

Who reads Kaempferol Protects Mitochondria and Alleviates Damages Against Endotheliotoxicity Induced by Doxorubicin?

Author

Wu, Weiqi; Yang, Bin; Qiao, Yang; Zhou, Qing; He, Huan; He, Ming

Publisher

Elsevier Science; Elsevier ; Elsevier Masson; Elsevier BV (ISSN 0753-3322)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)