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β-carotene improves fecal dysbiosis and intestinal dysfunctions in a mouse model of vitamin A deficiency 1 1 Introduction 1 2 Material and methods 2 2.1 Mice, diet and β-carotene supplementation 2 2.2 HPLC analysis of retinoids 2 2.3 RNA extraction and quantitative real-time PCR (QPCR) 2 2.4 Preparation of hydro-indocyanine green (H-ICG) and in vivo ROS imaging and analysis 2 2.5 Fecal sample collection and DNA extraction 3 2.6 16S rRNA library construction and sequencing 3 2.7 Informatics analysis 3 2.8 Statistical analysis 3 3 Results 3 3.1 β-carotene as the sole dietary source of vitamin A ameliorates retinoid concentrations in serum and tissues of the vita ... 3 3.2 β-carotene supplementation alters the taxonomic profile of the fecal microbiome of the vitamin A deficient Lrat−/-Rbp−/ ... 4 3.3 Dietary β-carotene impacts the physical and functional barrier properties of the colon 5 4 Discussion 6 5 Conclusions 10 CRediT authorship contribution statement 10 Declaration of competing interest 11 Acknowledgments 11 Data availability 11 Appendix A Supplementary data 11 References 11

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EN

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nonfiction