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Validated Assay for Quantification of Oxcarbazepine and Its Active Dihydro Metabolite 10-Hydroxycarbazepine in Plasma by Atmospheric Pressure Chemical Ionization Liquid Chromatography/mass Spectrometry by Hans H. Maurer; Carsten Kratzsch; Armin A. Weber; Frank T. Peters; Thomas Kraemer is a Chemistry article available to read on EtoBox.

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## Abstract Oxcarbazepine (OX), a new antiepileptic, may lead to unwanted side‐effects or even life‐threatening intoxications after overdose. Therefore, a validated liquid chromatographic/mass spectrometric (LC/MS) assay was developed for the quantification of OX and its pharmacologically active dihydro metabolite (dihydrooxcarbazepine, DOX, often named 10‐hydroxycarbazepine). OX and DOX were extracted from plasma by the authors' standard liquid/liquid extraction and were separated on a Merck LiChroCART column with Superspher 60 RP Select B as the stationary phase. Gradient elution was performed using aqueous ammonium formate and acetonitrile. The compounds were quantified in the selected‐ion monitoring mode using atmospheric pressure chemical ionization electrospray LC/MS. The assay was fully validated. It was found to be selective. The calibration curves were linear from 0.1 to 50 mg l^−1^ for OX and DOX. Limits of quantification were 0.1 mg l^−1^ for OX and DOX. The absolute recoveries were between 60 and 86%. The accuracy and precision data were within the required limits. The analytes in frozen plasma samples were stable for at least 1 month. The method was successfully applie

Who reads Validated Assay for Quantification of Oxcarbazepine and Its Active Dihydro Metabolite 10-Hydroxycarbazepine in Plasma by Atmospheric Pressure Chemical Ionization Liquid Chromatography/mass Spectrometry?

Author

Hans H. Maurer; Carsten Kratzsch; Armin A. Weber; Frank T. Peters; Thomas Kraemer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)