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Quantitative determination of MDR1 mRNA expression in peripheral blood lymphocytes: a possible role of genetic polymorphisms in the MDR1 gene by K. Oselin; I. Nowakowski‐Gashaw; P. M. Mrozikiewicz; D. Wolbergs; R. Pähkla; I. Roots is a Medicine article available to read on EtoBox.
What is Quantitative determination of MDR1 mRNA expression in peripheral blood lymphocytes: a possible role of genetic polymorphisms in the MDR1 gene about?
## Abstract **Background** P‐glycoprotein is a transmembrane efflux pump that extrudes a wide variety of drugs, thereby reducing their intracellular access. In humans, P‐glycoprotein is encoded by the MDR1 gene. Recently, several single nucleotide polymorphisms in the MDR1 gene were identified. Moreover, it was postulated that, in addition to the full‐length P‐glycoprotein, a ‘mini’ P‐glycoprotein was also present in lymphocytes. **Materials and methods** We investigated the effect of the genetic polymorphisms G2677T and C3435T in the MDR1 gene on MDR1 mRNA expression in FACS‐sorted peripheral blood CD4^+^, CD8^+^, CD19^+^, and CD56^+^ cells. MDR1 mRNA expression was determined in 45 healthy individuals using a real‐time quantitative RT‐PCR. **Results** We detected the highest expression of MDR1 mRNA in CD56^+^ cells, followed by CD8^+^ > CD4^+^ > CD19^+^ cells. However, genetic polymorphisms of the MDR1 gene failed to affect (__P__ > 0·05) MDR1 mRNA levels in the peripheral blood lymphocytes. Furthermore, the transcript levels for the MDR1 N‐terminal half were almost two‐fold lower than that of the MDR1 C‐terminal half in all cell populations investigated (__P__ < 0·0001). **Concl
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- Author
- K. Oselin; I. Nowakowski‐Gashaw; P. M. Mrozikiewicz; D. Wolbergs; R. Pähkla; I. Roots
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0014-2972)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)