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Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes by Kunal Jindal; Mohd Tayyab Adil; Naoto Yamaguchi; Xue Yang; Helen C. Wang; Kenji Kamimoto; Guillermo C. Rivera-Gonzalez; Samantha A. Morris is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes about?

## Abstract Complex gene regulatory mechanisms underlie differentiation and reprogramming. Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that enables direct capture of heritable random barcodes expressed as polyadenylated transcripts, in both single-cell RNA sequencing and single-cell Assay for Transposase Accessible Chromatin using sequencing assays, allowing for independent clonal tracking of transcriptional and epigenomic cell states. We validate CellTag-multi to characterize progenitor cell lineage priming during mouse hematopoiesis. Additionally, in direct reprogramming of fibroblasts to endoderm progenitors, we identify core regulatory programs underlying on-target and off-target fates. Furthermore, we reveal the transcription factor Zfp281 as a regulator of reprogramming outcome, biasing cells toward an off-target mesenchymal fate. Our results establish CellTag-multi as a lineage-tracing method compatible with multiple single-ce

Who reads Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes?

Author

Kunal Jindal; Mohd Tayyab Adil; Naoto Yamaguchi; Xue Yang; Helen C. Wang; Kenji Kamimoto; Guillermo C. Rivera-Gonzalez; Samantha A. Morris

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)