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Collagenase Treatment of Cartilaginous Matrix Promotes Fusion of Adjacent Cartilage by Ching-Chuan Jiang; Chang-Hsun Hsieh; Chun-Jen Liao; Wen-Hsiang Chang; Wei-Ju Liao; Jyy-Jih Tsai-Wu; Hongsen Chiang is a Medicine article available to read on EtoBox.

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In articular cartilage-repair, grafts usually fuse unsatisfactorily with surrounding host cartilage. Enzymatic dissociation of cartilaginous matrix to free chondrocytes may benefit fusion. We tested such a hypothesis with human cartilage __in vitro__, and with porcine cartilage __in vivo__. Human articular cartilage was collected from knee surgeries, cut into disc-and-ring sets, and randomly distributed into three groups: disc-and-ring sets in Group 1 were left untreated; in Group 2 only discs, and in Group 3 both discs and rings were treated with enzyme. Each disc-and-ring reassembly was cultured in a perfusion system for 14 days; expression of cartilage marker proteins and genes was evaluated by immunohistochemistry and PCR. Porcine articular cartilage from knees was similarly fashioned into disc-and-ring combinations. Specimens were randomly distributed into a control group without further treatment, and an experimental group with both disc and ring treated with enzyme. Each disc-and-ring reassembly was transplanted into subcutaneous space of a nude mouse for 30 days, and retrieved to examine disc-ring interface. In __in vitro__ study with human cartilage, a visible gap remained

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Author

Ching-Chuan Jiang; Chang-Hsun Hsieh; Chun-Jen Liao; Wen-Hsiang Chang; Wei-Ju Liao; Jyy-Jih Tsai-Wu; Hongsen Chiang

Publisher

Elsevier BV

Published

2020

Language

EN

Field

Medicine (Health Sciences)