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Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK) by Miriam Sannomiya; Clenilson M Rodrigues; Roberta G Coelho; Lourdes C dos Santos; Clélia A Hiruma-Lima; Alba R.M Souza Brito; Wagner Vilegas is a Chemistry article available to read on EtoBox.
What is Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK) about?
The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-␣-l-arabinoside, quercetin-3-O--d-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-␣-l-arabinoside (95 mg), quercetin-3-O--d-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1 H NMR, 13 C NMR and UV analyses.
Who reads Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Miriam Sannomiya; Clenilson M Rodrigues; Roberta G Coelho; Lourdes C dos Santos; Clélia A Hiruma-Lima; Alba R.M Souza Brito; Wagner Vilegas
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)