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Identification of a Pentaketide Stilbene Produced by a Type III Polyketide Synthase from Pinus sylvestris and Characterisation of Free Coenzyme A Intermediates by Tsung‐Lin Li; Dieter Spiteller; Jonathan B. Spencer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification of a Pentaketide Stilbene Produced by a Type III Polyketide Synthase from Pinus sylvestris and Characterisation of Free Coenzyme A Intermediates about?

## Abstract **By miscounting** the number of malonyl‐CoA condensations, the stilbene synthase (STS) from __Pinus sylvestris__ forms the previously unknown pentaketide, 2‐malonylresveratrol, in addition to the expected tetraketide resveratrol (see scheme). This is the first time that such tetra‐ and pentaketide‐CoA derivatives have been observed; this suggests that these products might be free intermediates in the biosynthesis of stilbenes.magnified image __The stilbene synthase (STS) from Scots pine (__Pinus sylvestris__), which is a type III polyketide synthase, produces the well known tetraketide resveratrol from coumaroyl‐CoA and three molecules of malonyl‐CoA. The same stilbene synthase, however, also generates the previously unknown pentaketide 2‐malonylresveratrol from coumaroyl‐CoA and four molecules of malonyl‐CoA; this indicates that the enzyme does not precisely control the number of condensations leading to diverse products. Tetraketide‐ and pentaketide‐CoA intermediates of the STS were identified by LC‐MS/MS; this suggests that CoA‐bound polyketide intermediates are involved in the product formation of type III polyketide synthases.__

Who reads Identification of a Pentaketide Stilbene Produced by a Type III Polyketide Synthase from Pinus sylvestris and Characterisation of Free Coenzyme A Intermediates?

Author

Tsung‐Lin Li; Dieter Spiteller; Jonathan B. Spencer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1439-4227)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)