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Functionalised ethylene vinyl alcohol copolymer (EVAL) membranes for affinity protein separation by M.E. Avramescu; W.F.C. Sager; M. Wessling is a Engineering article available to read on EtoBox.

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Hydrophilic microfiltration membranes with functional groups that can be used as coupling sites for ligands are of central interest in affinity separation, especially in view of biomedical applications. In this study we investigate covalent coupling of bovine serum albumin (BSA) as model ligand onto cellular-type poly(ethylene vinyl alcohol) (EVAL) microfiltration membranes. EVAL membranes prepared from the ternary water/DMSO/EVAL system are only suitable for activation and coupling reactions in aqueous media. Employing glutaraldehyde (GA) or oxiran to activate the secondary alcohol groups of the vinyl alcohol segments yielded a BSA-immobilization per internal area of 0.1-0.2 g/cm 2 (4-8 mg/g per membrane mass). Preparing microfiltration membranes from the quaternary water/1-octanol/DMSO/EVAL system, using 1-octanol as nonsolvent-additive in the casting solution, enabled to perform surface functionalization reactions in organic media as well as surface activation by a low-pressure glow discharge treatment. 0.3-0.45 g/cm 2 BSA per internal area (16-18 mg/g per membrane mass) was covalently coupled onto the porous membranes by applying trichloro-s-triazine (sTT) or sulfonyl chloride

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Author

M.E. Avramescu; W.F.C. Sager; M. Wessling

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0376-7388)

Published

2003

Language

EN

Field

Engineering (Physical Sciences)