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The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis by Qian Gao; Ranjha Khan; Changping Yu; Manfred Alsheimer; Xiaohua Jiang; Hui Ma; Qinghua Shi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis about?
Sperm head shaping is a key event in spermiogenesis and is tightly controlled via the acrosome–manchette network. Linker of nucleoskeleton and cytoskeleton (LINC) complexes consist of Sad1 and UNC84 domain–containing (SUN) and Klarsicht/ANC-1/Syne-1 homology (KASH) domain proteins and form conserved nuclear envelope bridges implicated in transducing mechanical forces from the manchette to sculpt sperm nuclei into a hook-like shape. However, the role of LINC complexes in sperm head shaping is still poorly understood. Here we assessed the role of SUN3, a testis-specific LINC component harboring a conserved SUN domain, in spermiogenesis. We show that CRISPR/Cas9-generated Sun3 knockout male mice are infertile, displaying drastically reduced sperm counts and a globozoospermia-like phenotype, including a missing, mislocalized, or fragmented acrosome, as well as multiple defects in sperm flagella. Further examination revealed that the sperm head abnormalities are apparent at step 9 and that the sperm nuclei fail to elongate because of the absence of manchette microtubules and perinuclear rings. These observations indicate that Sun3 deletion likely impairs the ability of the LINC complex
Who reads The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Qian Gao; Ranjha Khan; Changping Yu; Manfred Alsheimer; Xiaohua Jiang; Hui Ma; Qinghua Shi
- Publisher
- The American Society for Biochemistry and Molecular Biology; American Society for Biochemistry and Molecular Biology; American Society for Biochemistry and Molecular Biology Inc.; American Society for Biochemistry & Molecular Biology (ASBMB); Elsevier BV (ISSN 0021-9258)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)