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Impact of sub-inhibitory antibiotics on fibronectin-mediated host cell adhesion and invasion by Staphylococcus aureus by Jean Philippe Rasigade; Abdelmalek Moulay; Yannick Lhoste; Anne Tristan; Michele Bes; François Vandenesch; Jerome Etienne; Gerard Lina; Frederic Laurent; Oana Dumitrescu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Impact of sub-inhibitory antibiotics on fibronectin-mediated host cell adhesion and invasion by Staphylococcus aureus about?
## Background __Staphylococcus aureus__ is a well-armed pathogen prevalent in severe infections such as endocarditis and osteomyelitis. Fibronectin-binding proteins A and B, encoded by __fnb__A/B, are major pathogenesis determinants in these infections through their involvement in __S. aureus__ adhesion to and invasion of host cells. Sub-minimum inhibitory concentrations (sub-MICs) of antibiotics, frequently occurring __in vivo__ because of impaired drug diffusion at the infection site, can alter __S. aureus__ phenotype. We therefore investigated their impact on __S. aureus__ fibronectin-mediated adhesiveness and invasiveness. ## Methods After __in vitro__ challenge of __S. aureus__ 8325-4 and clinical isolates with sub-MICs of major anti-staphylococcal agents, we explored __fnb__A/B transcription levels, bacterial adhesiveness to immobilised human fibronectin and human osteoblasts in culture, and bacterial invasion of human osteoblasts. ## Results Oxacillin, moxifloxacin and linezolid led to the development of a hyper-adhesive phenotype in the fibronectin adhesion assay that was consistent with an increase in __fnb__A/B transcription. Conversely, rifampin treatment decreased fibro
Who reads Impact of sub-inhibitory antibiotics on fibronectin-mediated host cell adhesion and invasion by Staphylococcus aureus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jean Philippe Rasigade; Abdelmalek Moulay; Yannick Lhoste; Anne Tristan; Michele Bes; François Vandenesch; Jerome Etienne; Gerard Lina; Frederic Laurent; Oana Dumitrescu
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2180)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)