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Cell Cycle Arrest Induced by Ectopic Expression of P27 Is Not Sufficient to Promote Oligodendrocyte Differentiation by X-M. Tang; J.S. Beesley; J.B. Grinspan; P. Seth; J. Kamholz; F. Cambi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Oligodendrocyte differentiation is accompanied by dramatic changes in gene expression as well as cell cycle arrest. To determine whether cell cycle arrest is sufficient to induce the changes in cell phenotype associated with differentiation, we inhibited oligodendrocyte precursor proliferation in vitro by overexpressing p27, a cyclin kinase inhibitor, using a recombinant adenovirus. Ectopic expression of p27 efficiently inhibited oligodendrocyte precursor cell division, even in the presence of exogenous mitogens, by blocking the activity of the cyclin-dependent kinase, cdk2. Although the cells had stopped dividing, they did not express galactocerebroside (GalC) or myelin basic protein (MBP), changes associated with oligodendrocyte differentiation, suggesting that they had not differentiated. After removal of exogenous mitogens, however, adenovirus-expressing oligodendrocyte precursors differentiated with a temporal profile similar to that of control, uninfected oligodendrocytes, as indicated by expression of GalC and MBP. We conclude that cell cycle arrest is not sufficient to induce differentiation of dividing oligodendrocyte precursors, and that modulation of additional, as yet u

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Author

X-M. Tang; J.S. Beesley; J.B. Grinspan; P. Seth; J. Kamholz; F. Cambi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)