About this document
Edman Degradation for Protein Sequencing by Adrian Pascual is a document available to read on EtoBox.
The document discusses the Edman degradation technique for determining the amino acid sequence of proteins. The technique involves treating the protein with phenylisothiocyanate to derivatize the N-terminus, then specifically cleaving off this derivative to reveal the next amino acid. This process can be repeated to sequentially determine the full sequence. However, peptides longer than around 50 amino acids cannot be fully sequenced using this method alone due to limitations, so enzymes are used to first b
- Author
- Adrian Pascual
- Language
- EN