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Membrane Filter-Deoxyribonucleic Acid Method of Somatic Cell Counting: Collaborative Study by Robert D Bremel; Collaborators:; T Bredar; R Case; B Cathardi; R Ellis; D Kroenke; V Marable; D Meyer; R Meyer; J Miller; T Rothering; G Schwinghammer; R Smith; J Tjornehoj is a Chemistry article available to read on EtoBox.
What is Membrane Filter-Deoxyribonucleic Acid Method of Somatic Cell Counting: Collaborative Study about?
## Abstract In the membrane filter-deoxyribonucleic acid test, somatic cells are trapped on a special filter. A chemical determination of the DNA of the trapped material gives an estimate of the number of cells. The MF-DNA was evaluated and compared with the direct microscopic somatic cell counts (DMSCCs) in 10 laboratories. Cell levels in the 10 bulk milk samples analyzed ranged from 380,000 to 1,700,000/mL. Regression analysis gave a relation of 0.181 absorbance at 490 nm, when 2.5 mL of milk was analyzed and 5.0 mL reagent was used for color development. The 95% confidence limits on the slope were 0.158-0.204. Analysis of variance showed that both procedures had significant (P<0.001) systematic errors, but that only the DMSCC method showed a significant laboratory X sample interaction (P<0.001). The repeatability standard deviation of the MF-DNA procedure was 117,000 cells/mL (14%), and the reproducibility standard deviation was 198,000/ mL (23%). This compared favorably with the DMSCC method which had a repeatability of I62,000/mL (19%) and a reproducibility of 287,000/mL (34%). The error of the MF-DNA was constant at all cell counts, whereas there was a tendency for the error
Who reads Membrane Filter-Deoxyribonucleic Acid Method of Somatic Cell Counting: Collaborative Study?
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- Author
- Robert D Bremel; Collaborators:; T Bredar; R Case; B Cathardi; R Ellis; D Kroenke; V Marable; D Meyer; R Meyer; J Miller; T Rothering; G Schwinghammer; R Smith; J Tjornehoj
- Publisher
- Association of Official Analytical Chemists; The Journal of AOAC International; AOAC International; Oxford University Press (OUP) (ISSN 1060-3271)
- Published
- 1980
- Language
- EN
- Field
- Chemistry (Physical Sciences)