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Expression and Functional Activity of Fibroblast Growth Factors and Their Receptors in Human Pancreatic Cancer by Hing Y. Leung; William J. Gullick; Nicholas R. Lemoine is a Medicine article available to read on EtoBox.

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## Abstract We have analysed expression of the first 7 members of the family of heparin‐binding fibroblast growth factor (FGFs) and their 4 high‐affinity receptors (FGFRs) in human pancreatic carcinoma cell lines, both at the mRNA and protein levels. In cell lines expressing FGFRs, 2 typical patterns were observed: (i) expression of FGFR‐1, −3 or −4 along with the expression of at least one FGF; (ii) co‐expression of FGFR‐3 and FGFR‐4 in the absence of FGF expression. Using RT‐PCR, transcripts representing multiple isoforms of both extracellular and intracellular domains of FGFR‐1 were detected in the cell line PT45. A novel extracellular domain variant of FGFR‐1 was predicted to encode the first immunoglobulin loop in a potentially secreted form. Protein expression of the splice variants of FGFR‐1 was confirmed by immunoprecipitation with specific antibodies in radiolabelled ligand cross‐linking experiments. The type I carboxyl end and the α subtype extracellular domain were detected in the PANC‐1 cell line, while the type I carboxyl terminus and the γ subtype extracellular domain were expressed in the PT45 cell line. Expression of FGF‐2 in PT45 was also detected by immunoprecipit

Who reads Expression and Functional Activity of Fibroblast Growth Factors and Their Receptors in Human Pancreatic Cancer?

Author

Hing Y. Leung; William J. Gullick; Nicholas R. Lemoine

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1994

Language

EN

Field

Medicine (Health Sciences)