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Functional Multimerization of Mucolipin Channel Proteins by Cyntia Curcio‐Morelli; Peng Zhang; Bhuvarahamurthy Venugopal; Florie A. Charles; Marsha F. Browning; Horacio F. Cantiello; Susan A. Slaugenhaupt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Functional Multimerization of Mucolipin Channel Proteins about?
## Abstract __MCOLN1__ encodes mucolipin‐1 (TRPML1), a member of the transient receptor potential TRPML subfamily of channel proteins. Mutations in __MCOLN1__ cause mucolipidosis‐type IV (MLIV), a lysosomal storage disorder characterized by severe neurologic, ophthalmologic, and gastrointestinal abnormalities. Along with TRPML1, there are two other TRPML family members, mucolipin‐2 (TRPML2) and mucolipin‐3 (TRPML3). In this study, we used immunocytochemical analysis to determine that TRPML1, TRPML2, and TRPML3 co‐localize in cells. The multimerization of TRPML proteins was confirmed by co‐immunoprecipitation and Western blot analysis, which demonstrated that TRPML1 homo‐multimerizes as well as hetero‐multimerizes with TRPML2 and TRPML3. MLIV‐causing mutants of TRPML1 also interacted with wild‐type TRPML1. Lipid bilayer re‐constitution of in vitro translated TRPML2 and TRPML3 confirmed their cation channel properties with lower single channel conductance and higher partial permeability to anions as compared to TRPML1. We further analyzed the electrophysiological properties of single channel TRPML hetero‐multimers, which displayed functional differences when compared to individual TR
Who reads Functional Multimerization of Mucolipin Channel Proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Cyntia Curcio‐Morelli; Peng Zhang; Bhuvarahamurthy Venugopal; Florie A. Charles; Marsha F. Browning; Horacio F. Cantiello; Susan A. Slaugenhaupt
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)