Can I read Challenges and strategies: Scalable and efficient production of mesenchymal stem cells-derived exosomes for cell-free therapy on EtoBox?

Challenges and strategies: Scalable and efficient production of mesenchymal stem cells-derived exosomes for cell-free therapy by Wei Pan; Hongyuan Chen; Aijun Wang; Fengshan Wang; Xinke Zhang is a Neuroscience article available to read on EtoBox.

What is Challenges and strategies: Scalable and efficient production of mesenchymal stem cells-derived exosomes for cell-free therapy about?

Exosomes are small membrane vesicles secreted by most cell types, and widely exist in cell supernatants and various body fluids. They can transmit numerous bioactive elements, such as proteins, nucleic acids, and lipids, to affect the gene expression and function of recipient cells. Mesenchymal stem cells (MSCs) have been confirmed to be a potentially promising therapy for tissue repair and regeneration. Accumulating studies demonstrated that the predominant regenerative paradigm of MSCs transplantation was the paracrine effect but not the differentiation effect. Exosomes secreted by MSCs also showed similar therapeutic effects as their parent cells and were considered to be used for cell-free regenerative medicine. However, the inefficient and limited production has hampered their development for clinical translation. In this review, we summarize potential methods to efficiently promote the yield of exosomes. We mainly focus on engineering the process of exosome biogenesis and secretion, altering the cell culture conditions, cell expansion through 3D dynamic culture and the isolation of exosomes. In addition, we also discuss the application of MSCs-derived exosomes as therapeutics

Who reads Challenges and strategies: Scalable and efficient production of mesenchymal stem cells-derived exosomes for cell-free therapy?

Author

Wei Pan; Hongyuan Chen; Aijun Wang; Fengshan Wang; Xinke Zhang

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Neuroscience (Life Sciences)