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Expression of herpes simplex virus type 2 US3 affects the Cdc42/Rac pathway and attenuates c‐Jun N‐terminal kinase activation by Takayuki Murata; Fumi Goshima; Tohru Daikoku; Hiroki Takakuwa; Yukihiro Nishiyama is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression of herpes simplex virus type 2 US3 affects the Cdc42/Rac pathway and attenuates c‐Jun N‐terminal kinase activation about?
Although the US3 gene product of herpes simplex virus (HSV) has been identified as a serine/threonine protein kinase (PK), the functions are poorly understood. We found that US3 PK of HSV‐2 induced disruption of actin filaments, cell rounding and accumulation of binucleate cells in HEp2 cells. Cell rounding was abrogated by expression of either kinase‐dead forms of US3 PK or a mutant protein lacking the acidic cluster in the kinase regulatory domain. Co‐expression of dominant active forms of Cdc42/Rac inhibited cell rounding, suggesting that a signal transduction pathway involving Cdc42/Rac may play a role in the morphological changes induced by the kinase. Cdc42 and Rac, members of the Rho family of small GTPases, function as molecular switches changing actin cytoskeletal organization, influencing transcription and controlling apoptotic cell death. By computed homology search, we noticed significant similarities between US3 PK and p21‐activated kinase (PAK), which is activated by the Cdc42 or Rac. We also found that the expression of US3 suppressed the activation of c‐Jun N‐terminal kinase (JNK), a kinase that is downstream of PAK. These observations suggest that the US3 PK affect
Who reads Expression of herpes simplex virus type 2 US3 affects the Cdc42/Rac pathway and attenuates c‐Jun N‐terminal kinase activation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Takayuki Murata; Fumi Goshima; Tohru Daikoku; Hiroki Takakuwa; Yukihiro Nishiyama
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)