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Further studies and biological activities of macromolecular protein R-Phycoerythrin from Portieria hornemannii by Namasivayam Senthilkumar; Chidambaram Kurinjimalar; Ramar Thangam; Veeraperumal Suresh; Ganapathy Kavitha; Palani Gunasekaran; Ramasamy Rengasamy is a Materials Science article available to read on EtoBox.

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In the present study, the purified R-Phycoerythrin (R-PE) from a red alga Portieria hornemannii was subjected to the analysis of stability under the influence of different agents. Among the various inhibitors tested on R-PE EDTA at lower concentrations (<1 mM) supported the activity of R-PE. When R-PE was exposed to different organic solvents, ethanol supported the activity at the maximum followed by acetone, ethyl acetate, chloroform and methanol. Citric acid, as a preservative maintained the stability of R-PE both under 0 ± 5 °C and 30 ± 5 °C with 59.34% and 56.23% respectively, on 30th day. Thermal decomposition of the R-PE began near 60 °C. Maximum weight loss was occurred between 150 °C and 500 °C. Complete weight loss was recorded around 875 °C. Thermal denaturation was observed between 19 °C and 40 °C. Moderate to low antioxidant activities were observed in R-PE in relation to total antioxidant activities. After characterization, R-PE was taken for in vitro anticancer studies against selected cancer cell lines. Further studies involving AO/EB fluorescence staining and phase contrast microscope revealed characteristic apoptotic features like cell shrinkage, membrane blebbing,

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Author

Namasivayam Senthilkumar; Chidambaram Kurinjimalar; Ramar Thangam; Veeraperumal Suresh; Ganapathy Kavitha; Palani Gunasekaran; Ramasamy Rengasamy

Publisher

Elsevier BV

Published

2013

Language

EN

Field

Materials Science (Physical Sciences)