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Silver Stain for Proteins on a Cellulose Acetate Membrane by Toshiko Fujita; Tosifusa Toda; Mochihiko Ohashi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Silver Stain for Proteins on a Cellulose Acetate Membrane about?

A rapid and sensitive silver staining method to detect proteins on a cellulose acetate membrane has been established. This method is achieved by modification of the silver-based color staining for detection of proteins in polyacrylamide gels [D. W. Sammons, L. D. Adams, and E. E. Nishizawa, Electrophoresis 2, 135-141 (1981)] and applied to our new type of two-dimensional electrophoresis for analysis of proteins on a cellulose acetate sheet [T. Toda, T. Fujita, and M. Ohashi, Anal. Biochem. 119, 167-176 (1982)]. Maximal sensitivity of silver stain for proteins on a cellulose acetate membrane can be obtained by an optimal balance between deposition of silver on the protein and on the background. Certain kinds of proteins are colored red, orange, or grayish-blue. The silver stain is 20-80 times more sensitive than Coomassie blue and some spots are visualized reproducibly by silver only. Densitometric evaluation of standard proteins stained with silver and Coomassie blue is also demonstrated. The method takes only 50 min to perform and is sensitive, simple, and reproducible.

Who reads Silver Stain for Proteins on a Cellulose Acetate Membrane?

Author

Toshiko Fujita; Tosifusa Toda; Mochihiko Ohashi

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1984

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)