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Can I read Comparative Analysis of the Sequences and Three-Dimensional Models of Human Procarboxypeptidases A1, A2 and B on EtoBox?
Comparative Analysis of the Sequences and Three-Dimensional Models of Human Procarboxypeptidases A1, A2 and B by Patrick Aloy; Lluis Catasús; Virtudes Villegas; David Reverter; Josep Vendrell; Francesc X. Avilés is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Comparative Analysis of the Sequences and Three-Dimensional Models of Human Procarboxypeptidases A1, A2 and B about?
A full-length cDNA clone for preprocarboxypeptidase B from human pancreas has been isolated and sequenced. The open reading frame is 1254 bp in length, encoding a protein of 417 amino acids that includes a leader signal peptide of 15 amino acids and a 95-amino acid-long pro-segment. It contains two differences when compared to the sequence reported for pancreas-specific protein, a human serum marker for acute pancreatitis identified as procarboxypeptidase B. The main difference is a previously unreported Cys at position 138, which is needed for the formation of one of the three disulphide bridges. Sequence alignments between human procarboxypeptidases A1, A2 and B and other known forms show that the most conserved region is the enzyme moiety followed by the globular domain of the pro-segment. The maximum variability is found in the connecting region between moieties. The known three-dimensional structures of procarboxypeptidases from bovine and porcine species have been used to model all three human procarboxypeptidases and also to estimate the interaction energies between the different parts of the molecules, in an attempt to gain insight into the structural features responsible f
Who reads Comparative Analysis of the Sequences and Three-Dimensional Models of Human Procarboxypeptidases A1, A2 and B?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Patrick Aloy; Lluis Catasús; Virtudes Villegas; David Reverter; Josep Vendrell; Francesc X. Avilés
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)