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Lipopolysaccharide preferentially induces 4-1BB ligand expression on human monocyte-derived dendritic cells by Pao-Kung Lee; Chun-Jung Chang; Chun-Ming Lin is a Immunology and Microbiology article available to read on EtoBox.

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Dendritic cells (DCs) represent a promising tool for immunotherapy. A key feature in their action is to provide co-stimulatory signals for full activation of T cells. In view of recent studies demonstrating the critical role of 4-1BB co-stimulation in T cell response, it is of importance to optimize 4-1BB ligand (4-1BBL) expression on human monocyte-derived DCs (MDDCs), the DC source of many clinical studies. In this study, two types of MDDCs, generated in granulocyte-macrophage colony-stimulating factor and interleukin-4 (GM-CSF/IL-4-DCs) or in interferon-␤ and IL-3 (IFN-␤/IL-3-DCs), were analyzed for 4-1BBL expression in response to several known DC activators. Immature MDDCs expressed 4-1BBLs at very low levels. Lipopolysaccharide (LPS) was the only activator that preferentially triggered 4-1BBL expression on either MDDCs, but 4-1BBL-positive cells were significantly more frequently observed on LPS-activated GM-CSF/IL-4-DCs (30.2 ± 2.6% versus 14.3 ± 1.2%). Combinations of multiple activating signals did not bring about enhanced 4-1BBL stimulatory capacity. In addition, plasmid DNA transfection and necrotic cell pulsing of GM-CSF/IL-4-DCs for antigen loading also resulted in 4-1

Who reads Lipopolysaccharide preferentially induces 4-1BB ligand expression on human monocyte-derived dendritic cells?

Author

Pao-Kung Lee; Chun-Jung Chang; Chun-Ming Lin

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

2003

Language

EN

Field

Immunology and Microbiology (Life Sciences)