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gelatinase A/TIMP-2 imbalance in lymph-node-positive breast carcinomas, as measured by RT-PCR by Maurizio Onisto; Maria Pia Riccio; Paolo Scannapieco; Cristina Caenazzo; Luciano Griggio; Michele Spina; William G. Stetler-Stevenson; Spiridione Garbisa is a Medicine article available to read on EtoBox.

What is gelatinase A/TIMP-2 imbalance in lymph-node-positive breast carcinomas, as measured by RT-PCR about?

## Abstract Over‐production of gelatinase A (MMP‐2) or under‐production of its inhibitor (TIMP‐2) may result in the matrix degradation crucial for metastasis, and early evaluation of their expression in primary tumor would offer important prognostic informations. RT‐PCR amplicons of MMP‐2 and TIMP‐2 mRNA from tissue biopsies of 13 breast carcinomas and one fibrocystic mastopathy were quantitated. In comparison with their normal‐tissue counterparts, their expression trends were not uniform: in some cases MMP‐2 increased in the tumor without changes in TIMP‐2, in others TIMP‐2 expression also increased, although to a lesser extent than MMP‐2; only in 2 cases was it slightly lower in the tumor tissue. Nevertheless, clearer insights were gained from the comparison of the ratio (R) between MMP‐2~tumor/normal~ and TIMP‐2~tumor/norma~: as in the fibrocystic mastopathy, the R in carcinomas without lymph‐node involvement (LN^−^) was usually lower than 1 in most cases. In contrast, in 5 out of 6 patients with lymph‐node metastasis (LN^+^), the ratio ranged between 2 and 4. While the R magnitude was not related to the frequency of positive lymph nodes out of the total analyzed, nor to relapse

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Author

Maurizio Onisto; Maria Pia Riccio; Paolo Scannapieco; Cristina Caenazzo; Luciano Griggio; Michele Spina; William G. Stetler-Stevenson; Spiridione Garbisa

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1995

Language

EN

Field

Medicine (Health Sciences)