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Can I read Determination of Choline-containing Phospholipids in Serum with a Fiber-optic Biosensor on EtoBox?
Determination of Choline-containing Phospholipids in Serum with a Fiber-optic Biosensor by M.D. Marazuela; M.C. Moreno-Bondi is a Chemistry article available to read on EtoBox.
What is Determination of Choline-containing Phospholipids in Serum with a Fiber-optic Biosensor about?
An optical ®ber biosensor for choline-containing phospholipids monitoring in serum samples is described. Phospholipids are hydrolyzed by the enzyme phospholipase-D to choline which is analyzed with a ®ber-optic biosensor based on choline oxidase and oxygen transduction. Several supports have been evaluated for enzyme immobilization. The optimum biosensing layer consists of the enzyme immobilized on a nylon membrane and placed onto an oxygen sensitive layer formed by a luminescent Ru(II) complex, tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II), dispersed in a silicone membrane. The performance of the biosensor has been optimized using a ̄ow injection system. The linear dynamic range of the biosensing membranes is found to be 0.08±3.00 mg ml À1 phosphatidylcholine. Studies of the reproducibility, stability and interferences of the device, as well as the application of the sensor to measurements in serum samples, are reported.
Who reads Determination of Choline-containing Phospholipids in Serum with a Fiber-optic Biosensor?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- M.D. Marazuela; M.C. Moreno-Bondi
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1998
- Language
- EN
- Field
- Chemistry (Physical Sciences)