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Substrate specificity of new methyl-directed DNA endonuclease GlaI by Galina V Tarasova; Tatiana N Nayakshina; Sergey KH Degtyarev is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background Recently, we have discovered site-specific endonucleases, which recognize and cleave only DNA sequences with 5-methylcytosine. Two specificities of such endonucleases have been described. Enzymes BisI, BlsI, and GluI are isoschizomers and hydrolyze the DNA sequence 5'-GCNGC-3'/3'-CGNCG-5', which is methylated in different ways. The enzyme GlaI cleaves the DNA sequence 5'-GCGC-3'/3'-CGCG-5' if there are two, three or four 5-methylcytosines. The goal of the present work is to study in detail the composition of recognition sequence and effect of the methylated cytosines on the efficiency of DNA cleavage by the methyl-directed DNA endonuclease GlaI ## Results In a recent work we have studied the dependence of GlaI activity on the quantity and location of 5-methylcytosines in the enzyme recognition sequence 5'-GCGC-3'/3'-CGCG-5'. A significant DNA cleavage has been observed for oligonucleotide duplexes, which include either three or four 5-methylcytosines. In this work we have studied dependence of the GlaI activity on quantity and location of methylated cytosines, as well as on composition of the recognition sequence. ## Conclusion The list of good substrates for GlaI inc

Who reads Substrate specificity of new methyl-directed DNA endonuclease GlaI?

Author

Galina V Tarasova; Tatiana N Nayakshina; Sergey KH Degtyarev

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)