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This document provides instructions for using a uric acid assay to quantitatively determine uric acid levels in serum or plasma samples. Uric acid is oxidized by uricase and hydrogen peroxide is produced, which then reacts with 4-aminophenazone and dichlorophenol sulfonate to form a red quinoneimine compound. The intensity of the red color is proportional to the uric acid concentration in the sample. Normal uric acid levels in serum/plasma for men are 3.6-7.7 mg/dL and for women are 2.5-6.8 mg/dL. The assay
- Author
- Dafne Manrique Jerez
- Language
- EN