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Detection of Amyloid-β Fibrils Using Track-Etched Nanopores: Effect of Geometry and Crowding by Nathan Meyer; Nicolas Arroyo; Jean-Marc Janot; Mathilde Lepoitevin; Anna Stevenson; Imad Abrao Nemeir; Veronique Perrier; Daisy Bougard; Maxime Belondrade; Didier Cot; Jérémy Bentin; Fabien Picaud; Joan Torrent; Sebastien Balme is a Engineering article available to read on EtoBox.
What is Detection of Amyloid-β Fibrils Using Track-Etched Nanopores: Effect of Geometry and Crowding about?
Several neurodegenerative diseases have been linked to proteins or peptides that are prone to aggregate in different brain regions. Aggregation of amyloid-β (Aβ) peptides is recognized as the main cause of Alzheimer's disease (AD) progression, leading to the formation of toxic Aβ oligomers and amyloid fibrils. The molecular mechanism of Aβ aggregation is complex and still not fully understood. Nanopore technology provides a new way to obtain kinetic and morphological aspects of Aβ aggregation at a single-molecule scale without labeling by detecting the electrochemical signal of the peptides when they pass through the hole. Here, we investigate the influence of nanoscale geometry (conical and bullet-like shape) of a track-etched nanopore pore and the effect of molecular crowding (polyethylene glycol-functionalized pores) on Aβ fibril sensing and analysis. Various Aβ fibril samples that differed by their length were produced by sonication of fibrils obtained in the presence of epigallocatechin gallate. The conical nanopore functionalized with polyethylene glycol (PEG) 5 kDa is suitable for discrimination of the fibril size from . The bullet-like-shaped nanopore enhances the amplitude
Who reads Detection of Amyloid-β Fibrils Using Track-Etched Nanopores: Effect of Geometry and Crowding?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Nathan Meyer; Nicolas Arroyo; Jean-Marc Janot; Mathilde Lepoitevin; Anna Stevenson; Imad Abrao Nemeir; Veronique Perrier; Daisy Bougard; Maxime Belondrade; Didier Cot; Jérémy Bentin; Fabien Picaud; Joan Torrent; Sebastien Balme
- Publisher
- American Chemical Society (ACS)
- Published
- 2021
- Language
- EN
- Field
- Engineering (Physical Sciences)