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The growth response to androgen receptor signaling in ER**α**-negative human breast cells is dependent on p21 and mediated by MAPK activation by Joseph P Garay; Bedri Karakas; Abde M Abukhdeir; David P Cosgrove; John P Gustin; Michaela J Higgins; Hiroyuki Konishi; Yuko Konishi; Josh Lauring; Morassa Mohseni; Grace M Wang; Danijela Jelovac; Ashani Weeraratna; Cheryl A Sherman Baust; Patrice J Morin; Antoun Toubaji; Alan Meeker; Angelo M De Marzo; Gloria Lewis; Andrea Subhawong; Pedram Argani; Ben H Park is a Medicine article available to read on EtoBox.

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## Introduction Although a high frequency of androgen receptor (AR) expression in human breast cancers has been described, exploiting this knowledge for therapy has been challenging. This is in part because androgens can either inhibit or stimulate cell proliferation in pre-clinical models of breast cancer. In addition, many breast cancers co-express other steroid hormone receptors that can affect AR signaling, further obfuscating the effects of androgens on breast cancer cells. ## Methods To create better-defined models of AR signaling in human breast epithelial cells, we took estrogen receptor (ER)-α-negative and progesterone receptor (PR)-negative human breast epithelial cell lines, both cancerous and non-cancerous, and engineered them to express AR, thus allowing the unambiguous study of AR signaling. We cloned a full-length cDNA of human AR, and expressed this transgene in MCF-10A non-tumorigenic human breast epithelial cells and MDA-MB-231 human breast-cancer cells. We characterized the responses to AR ligand binding using various assays, and used isogenic MCF-10A p21 knock-out cell lines expressing AR to demonstrate the requirement for p21 in mediating the proliferative resp

Who reads The growth response to androgen receptor signaling in ER**α**-negative human breast cells is dependent on p21 and mediated by MAPK activation?

Author

Joseph P Garay; Bedri Karakas; Abde M Abukhdeir; David P Cosgrove; John P Gustin; Michaela J Higgins; Hiroyuki Konishi; Yuko Konishi; Josh Lauring; Morassa Mohseni; Grace M Wang; Danijela Jelovac; Ashani Weeraratna; Cheryl A Sherman Baust; Patrice J Morin; Antoun Toubaji; Alan Meeker; Angelo M De Marzo; Gloria Lewis; Andrea Subhawong; Pedram Argani; Ben H Park

Publisher

BioMed Central; Springer (Biomed Central Ltd.); Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1465-5411)

Published

2012

Language

EN

Field

Medicine (Health Sciences)