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Antibody Engineering (Springer Lab Manuals) by Stefan Dübel, Roland E. Kontermann (auth.), Dr. Roland Kontermann, Dr. Stefan Dübel (eds.) is a nonfiction available to read on EtoBox.

What is Antibody Engineering (Springer Lab Manuals) about?

Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided: Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radi

Who reads Antibody Engineering (Springer Lab Manuals)?

Author

Stefan Dübel, Roland E. Kontermann (auth.), Dr. Roland Kontermann, Dr. Stefan Dübel (eds.)

Publisher

Springer Berlin Heidelberg : Imprint: Springer

Published

2001

Language

EN

ISBN

9783662046050

Category

nonfiction

Subjects

Technology, Science, Chemistry