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FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism by Sneha Shah; Gemma Molinaro; Botao Liu; Ruijia Wang; Kimberly M. Huber; Joel D. Richter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism about?
## SUMMARY Silencing of **FMR1** and loss of its gene product, **FMRP**, results in fragile X syndrome (FXS). FMRP binds brain m**RNA**s and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in **__Fmr1__**-deficient tissue mostly reflect changes in **RNA** abundance. Profiling over a time course of ribosome runoff in wild-type tissue reveals a wide range of ribosome translocation rates; on many m**RNA**s, the ribosomes are stalled. Sucrose gradient ultracentrifugation of hippocampal slices after ribosome runoff reveals that **FMRP** co-sediments with stalled ribosomes, and its loss results in decline of ribosome stalling on specific m**RNA**s. One such m**RNA** encodes **SETD2**, a lysine methyltransferase that catalyzes H3K36me3. Chromatin immunoprecipitation sequencing (ChIP-seq) demonstrates that loss of **FMRP** alters the deployment of this histone mark. H3K36me3 is associated with alternative pre**-RNA** processing, which we find occurs in an **FMRP**-dependent manner on transcripts linked to neural function and autism spectrum disorders.
Who reads FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sneha Shah; Gemma Molinaro; Botao Liu; Ruijia Wang; Kimberly M. Huber; Joel D. Richter
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)