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Recycling Immunoaffinity Chromatography for Multiple Analyte Analysis in Biological Samples by Terry M Phillips; Janette M Krum is a Chemistry article available to read on EtoBox.
What is Recycling Immunoaffinity Chromatography for Multiple Analyte Analysis in Biological Samples about?
The ability to isolate and measure multiple complex analytes in a single biological sample holds great potential in many biomedical fields, especially immunology and diagnostic clinical chemistry. We have developed a procedure involving recycling immunoaffinity chromatography for the simultaneous measurement of a number of analytes in a single sample. The procedure is based on the passage of a fluorochrome-labelled sample through a battery of small immunoaffinity columns, each column extracting a single analyte. Detection is achieved by acid elution of the bound analytes and laser-induced fluorescence. We have applied this system to a number of different biological fluids and found that it is capable of reliably isolating and measuring up to ten different cytokines in a 25-ml sample of human body fluid.
Who reads Recycling Immunoaffinity Chromatography for Multiple Analyte Analysis in Biological Samples?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Terry M Phillips; Janette M Krum
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 1998
- Language
- EN
- Field
- Chemistry (Physical Sciences)