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LncRNA NEAT1/miR-128-3p/AQP4 axis regulating spinal cord injury-induced neuropathic pain progression by Shuyue Xian; Ruiwen Ding; Mengyun Li; Feng Chen is a Medicine article available to read on EtoBox.

What is LncRNA NEAT1/miR-128-3p/AQP4 axis regulating spinal cord injury-induced neuropathic pain progression about?

## Background: Neuropathic pain (np) is the comorbidity in spinal cord injury(sci), which is the hardest to cure. non-coding rna dysregulations are related to the development of np. neat1(nuclear paraspeckle assembly transcript 1) is a new type of lncrna. this study explores the role and specific mechanism of neat1 in sci-mediated np. ## Methods: Firstly, the neat1 expression in sci rats and the control group was detected with rt-pcr to analyze the relationship between neat13 and np symptoms. then, sci rats were intrathecally injected with neat13 overexpressing and knocking down lentiviruses. afterward, elisa was utilized to assess the expression of il-6, il-1β and tnfα in rats. subsequently, immunohistochemistry was adopted to verify the activation of microglial cells. after that, bioinformatics analysis was employed to further predict the downstream target genes of neat1, while rt-pcr and western blot were conducted to determine the relative expression of mir-128-3p and aquaporin-4(aqp4). meanwhile, a dual-luciferase reporter assay was performed to further study the targeting relationship between neat1 and mir-128-3p, and mir-128-3p and aqp4. ## Results: Sci rats showed distinctl

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Author

Shuyue Xian; Ruiwen Ding; Mengyun Li; Feng Chen

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-5728)

Published

2020

Language

EN

Field

Medicine (Life Sciences)