Can I read Engineering mRNA stability inE. coli by the addition of synthetic hairpins using a 5′ cassette system on EtoBox?
Engineering mRNA stability inE. coli by the addition of synthetic hairpins using a 5′ cassette system by Trent A. Carrier; J. D. Keasling is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Engineering mRNA stability inE. coli by the addition of synthetic hairpins using a 5′ cassette system about?
An expression system has been developed for the introduction of DNA cassettes into the region between the transcription and translation start sites of a gene of interest. This cassette system was used to engineer mRNA stability through the introduction of hairpins at the 5Ј end. A synthetic DNA cassette was designed so that the resulting mRNA hairpin would be positioned one nucleotide from the 5Ј mRNA end. The hairpincontaining mRNA exhibited a half-life 3 times that of the mRNA with no hairpin, resulting in increases in both mRNA and protein levels. These results indicate that it is possible to engineer mRNA stability as an additional means of controlling gene expression.
Who reads Engineering mRNA stability inE. coli by the addition of synthetic hairpins using a 5′ cassette system?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Trent A. Carrier; J. D. Keasling
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)