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Glycosidase mechanisms: Sugar conformations and reactivity in endo- and exo-acting enzymes by Mariana Abrahão Bueno Morais; Alba Nin-Hill; Carme Rovira is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Glycosidase mechanisms: Sugar conformations and reactivity in endo- and exo-acting enzymes about?

The enzymatic breakdown of carbohydrates plays a critical role in several biological events and enables the development of sustainable processes to obtain bioproducts and biofuels. In this scenario, the design of efficient inhibitors for glycosidases that can act as drug targets and the engineering of carbohydrate-active enzymes with tailored catalytic properties is of remarkable importance. To guide rational approaches, it is necessary to elucidate enzyme molecular mechanisms, in particular understanding how the microenvironment modulates the conformational space explored by the substrate. Computer simulations, especially those based on ab initio methods, have provided a suitable atomic description of carbohydrate conformations and catalytic reactions in several glycosidase families. In this review, we will focus on how the active-site topology (pocket or cleft) and mode of cleavage (endo or exo) can affect the catalytic mechanisms adopted by glycosidases, in particular the substrate conformations along the reaction coordinate.

Who reads Glycosidase mechanisms: Sugar conformations and reactivity in endo- and exo-acting enzymes?

Author

Mariana Abrahão Bueno Morais; Alba Nin-Hill; Carme Rovira

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)